MultiFlow DNA Damage Kit – Cleaved PARP, γH2AX, Phospho-Histone H3 Kit

Analyze in vitro samples for DNA damage using your own flow cytometer

Study multiple endpoints associated with DNA damage response pathways. These endpoints include cleaved PARP as an apoptosis marker, yH2AX as a measure of DNA double strand breaks, and phospho-histone H3 to label mitotic cells.

Product ID: MultiFlow (PARP, γH2AX, H3)

Would you like to add micronucleus data to your study?

Click here for the MicroFlow In Vitro 2,000/400 kit.

Would you like to measure mode of action in human cell lines?

Click here for the MultiFlow (p53, γH2AX, H3) kit.

Would you like to measure aneugens and clastogens at 24 hours?

Click here for the MultiFlow (p53) kit.

Technical Specifications

Size of Kit

384 tests

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • Nuclei Release Solution with Counting Beads
    Lyses cells and provides absolute bead count
  • DNA Stain
    Labels free nuclei
  • RNase Solution
    Removes RNA
  • Cleaved PARP Antibody FITC
    Detects apoptotic chromatin
  • γH2AX Antibody Alexa Fluor® 647
    Detects double-strand breaks
  • Phospho-Histone H3 Antibody PE
    Detects mitotic cells

Additional Materials Required

  • Cell line and cell culture materials
  • Refrigerator (4 °C)
  • Centrifuge and other general lab supplies
  • Accutase® cell detachment solution if working with attachment cells
  • Flow cytometer capable of 488 nm and 647 nm excitation, optionally equipped with High Throughput Sampler (HTS) or similar autosampler

Resources

Publications Q & A Support