In Vivo Liver Micronucleus Analysis

 

(MicroFlow® Kits)

Measuring DNA Damage
in Liver Hepatocytes

Exposure to a test substance can result in damage to the chromosomes or spindle apparatus of cells. During routine cell division, this type of damage can create a smaller ‘micro’-nucleus, apart from the main nucleus.

Liver hepatocytes in young animals proliferate at a high rate. Micronucleus formation in hepatocytes is a well-established marker of chromosomal damage. This makes assessment of micronuclei in liver a valuable endpoint.

In Vivo Liver MicroFlow kits use flow cytometry to quickly and reproducibly measure micronuclei.


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Features

  • Established Method
    A large number of chemicals have been evaluated with this automated method.
  • Flow Cytometry
    Fast, reproducible results that take advantage of laser-based technologies.
  • Analysis Services Available
    Send us your liver specimens and we'll take care of the processing and analysis.
  • QC’d Kits, Unlimited Technical Support
    Everything you need to successfully perform the method is included in the box or available for download!

Benefits

  • Regulatory Guideline Being Prepared
    An OECD Test Guideline submission is underway for the liver micronucleus assay.
  • Complete More Studies in Less Time
    Routinely score 5,000 cells per sample and analyze an entire study in one day!
  • Obtain Reproducible Data
    Objective flow cytometric software ensures robust, repeatable data.
  • Feel Confident
    Speak with the scientists who developed this method. Send plots, email, or call with questions!

Species Compatibility

Can be applied to mouse or rat species

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Advantages of Liver

Enables investigation of compounds that are only active at site of metabolism
•
Readily integrates with other methods
 

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Unlimited Technical Support

Speak with the scientists who designed the method
•
Unlimited phone and email technical support
(send us your plots!)
•
Training, at your site or ours

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Liver Micronucleus Method in 3Rs Friendly Study Design

We developed an In Vivo Liver Micronucleus method that uses automated flow cytometry to obtain this important chromosome damage endpoint and combined it with a 3Rs friendly study design.

Speak with the scientists who designed the method


 
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Flow Cytometer Template

Flow Cytometer Template - PLUS Rat Liver - For BD FACSDiva™ software Flow Cytometer Template - PLUS Mouse Liver - For BD FACSDiva™ software

Instruction Manual

Instruction Manual - MicroFlow PLUS Rodent Liver

Safety Data Sheet (SDS)

Safety Data Sheets (SDS) - MicroFlow PLUS Rodent Liver

Product Sheets

About Litron