In Vitro Micronucleus Analysis

(MicroFlow® Kits)

Measuring DNA Damage
in Cell Cultures

Exposure to a test substance can result in damage to the chromosomes or spindle apparatus of cells. During routine cell division, this type of damage can create a smaller ‘micro’-nucleus, apart from the main nucleus.

The key element of this method is the sequential staining. One staining step identifies dead and dying cells. The second step lyses cells and stains DNA. This approach allows you to distinguish micronuclei from other events, such as apoptotic bodies.

In Vitro MicroFlow kits use flow cytometry to quickly and reproducibly measure micronuclei.

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Features

  • Automated Scoring by Flow Cytometry
    Templates and example data are provided.
  • Compatible with Many Cell Lines
    With CHO-K1 cells, obtain frequency of hypodiploid nuclei.
  • Miniaturized Format
    Compatible with 96 well plates, as well as autosamplers and robotics.
  • QC’d Kits, Unlimited Technical Support
    Everything you need to successfully perform the method is included in the box or available for download!

Benefits

  • Reliable and Reproducible Data
    Reduce errors and get objective results you can trust.
  • Detects Multiple Modes of Action
    Determine if your compounds are aneugenic or clastogenic.
  • Reduces Time & Compound Requirements
    Hundreds of samples analyzed in a few hours. Eliminates tech time with "walk away" analysis.
  • Feel Confident
    Speak with the scientists who developed this method. Send plots, email, or call with questions!

Attachment or Suspension Cells

L5178Y
•
CHO-K1
•
TK6
•
V79
•
WIL-2
•
HepG2

High Content Analysis

Mode of Action
•
Cell cycle
•
Relative survival
•
Membrane integrity
 

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ICH Guidelines

In vitro micronucleus results are now accepted for
GLP submissions as a part of the
standard battery of genotoxicity tests.

This change was part of the effort to 
reduce the number of irrelevant positives for
in vitro mammalian assays.

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Unlimited Technical Support

Speak with the scientists who designed the method
•
Unlimited phone and email technical support
(send us your plots!)
•
Training, at your site or ours

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Flow Cytometer Template

Flow Cytometer Template - In Vitro MicroFlow - For BD CellQuest™ v3.3 software Flow Cytometer Template - In Vitro MicroFlow - For BD CellQuest™ Pro v5.2 software Flow Cytometer Template - In Vitro MicroFlow - For BD FACSDiva™ v6.1.1 software Flow Cytometer Template - In Vitro MicroFlow - For MACSQuant® software

Instruction Manual

Instruction Manual - In Vitro MicroFlow - For treatment in 96-well plates Instruction Manual - In Vitro MicroFlow - For treatment in flasks, or in larger format plates

On-Demand Videos

LEARN 101 In Vitro MicroFlow

Safety Data Sheet (SDS)

Safety Data Sheets (SDS) - In Vitro MicroFlow

Setup or Example Data

Example Data - In Vitro MicroFlow - For BD CellQuest™ Pro v5.2 and CellQuest™ Pro v3.3 software Example Data - In Vitro MicroFlow - For BD FACSDiva™ v6.1.1 software

Training Videos (Password Required)

Reagent Preparation Suspension Cell Protocol

Product Sheets

About Litron In Vitro Micronucleus Analysis Advantages of Flow Cytometry