In Vivo Micronucleus Analysis

 

(MicroFlow Kits)

Measuring DNA Damage
in Red Blood Cells

Exposure to a test substance can result in damage to the chromosomes or spindle apparatus of cells. During routine cell division, this type of damage can create a smaller ‘micro’-nucleus, apart from the main nucleus.

When red blood cells mature the main nucleus is expelled. Micronuclei remain behind and can easily be seen in a cell with no other DNA. This makes red blood cells ideal for measuring this endpoint.

In Vivo MicroFlow kits use flow cytometry to quickly and reproducibly measure micronuclei.

OECD Guideline 474

The required number of immature erythrocytes
scored has increased from 2,000 to 4,000.

Rat blood is formally accepted,
allowing for routine integration.

Flow cytometry and other automated
methods are now recommended.

Learn More

Unlimited Technical Support

Speak with the scientists who designed the method

Unlimited phone and email technical support
(send us your plots!)

Training, at your site or ours

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Features

  • Gold Standard Method
    A large number of chemicals has been evaluated with this internationally validated method.
  • Flow Cytometry
    Fast, reproducible results that take advantage of laser-based technologies.
  • Includes Calibration Standards
    Malaria Biostandards, with Positive and Negative Controls, ensure proper flow cytometric setup.
  • QC’d Kits, Unlimited Technical Support
    Everything you need to successfully perform the method is included in the box or available for download!

Benefits

  • Accepted by Regulatory Agencies
    GLP studies can be performed either at your facility or using Litron’s experienced scientists.
  • Complete More Studies in Less Time
    Routinely score 20,000 cells per sample and analyze an entire study in one day!
  • Provides Reproducible Data
    Calibration standards ensure confidence in your results across days and between laboratories.
  • Feel Confident
    Speak with the scientists who developed this method. Send plots, email, or call with questions!

Species and Compartments

Mouse or Rat

Blood or Bone Marrow

For others species, please inquire

Advantages of Peripheral Blood

Allows each subject to be sampled multiple times

Easily integrates into existing toxicology studies

Requires very low sample volumes

Reduces the number of animals in your studies

 

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