In Vivo Micronucleus Analysis

 

(MicroFlow® Kits)

Measuring DNA Damage
in Red Blood Cells

Exposure to a test substance can result in damage to the chromosomes or spindle apparatus of cells. During routine cell division, this type of damage can create a smaller ‘micro’-nucleus, apart from the main nucleus.

When red blood cells mature the main nucleus is expelled. Micronuclei remain behind and can easily be seen in a cell with no other DNA. This makes red blood cells ideal for measuring this endpoint.

In Vivo MicroFlow kits use flow cytometry to quickly and reproducibly measure micronuclei.

Features

  • Gold Standard Method
    A large number of chemicals have been evaluated with this internationally validated method.
  • Flow Cytometry
    Fast, reproducible results that take advantage of laser-based technologies.
  • Includes Calibration Standards
    Malaria Biostandards, with Positive and Negative Controls, ensure proper flow cytometric setup.
  • QC’d Kits, Unlimited Technical Support
    Everything you need to successfully perform the method is included in the box or available for download!

Benefits

  • Accepted by Regulatory Agencies
    GLP studies can be performed either at your facility or using Litron’s experienced scientists.
  • Complete More Studies in Less Time
    Routinely score 20,000 cells per sample and analyze an entire study in one day!
  • Provides Reproducible Data
    Calibration standards ensure confidence in your results across days and between laboratories.
  • Feel Confident
    Speak with the scientists who developed this method. Send plots, email, or call with questions!

Species and Compartments

Mouse or Rat
•
Blood or Bone Marrow
•
For others species, please inquire

Advantages of Peripheral Blood

Allows each subject to be sampled multiple times
•
Easily integrates into existing toxicology studies
•
Requires very low sample volumes
•
Reduces the number of animals in your studies

 

View Kits

OECD Guideline 474

The required number of immature erythrocytes
scored has increased from 2,000 to 4,000.
•
Rat blood is formally accepted,
allowing for routine integration.
•
Flow cytometry and other automated
methods are now recommended.

Learn More

Unlimited Technical Support

Speak with the scientists who designed the method
•
Unlimited phone and email technical support
(send us your plots!)
•
Training, at your site or ours

Learn More

Excel Spreadsheets

Flow Cytometer Template

Instruction Manual

On-Demand Videos

Safety Data Sheet (SDS)

Sample Submission Form

Setup or Example Data

Study Phase Plan

Training Videos (Password Required)

Product Sheets

Alternative Fixing Procedure