Significant changes from previous version

The required number of immature erythrocytes scored has increased from 2,000 to 4,000
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Automated scoring platforms are approved and recommended (e.g., flow cytometry)
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Rat blood is formally accepted
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More flexibility in dosing and sampling times
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Concurrent positive controls no longer required

Automated scoring methods are accepted and recommended (e.g., flow cytometry)

OECD updated guideline 474 states, "Counting sufficient immature erythrocytes in the peripheral blood or bone marrow of adult animals is greatly facilitated by using an automated scoring platform."

Also, "Comparative studies have shown that such methods, using appropriate calibration standards, can provide better inter- and intra-laboratory reproducibility and sensitivity than manual microscopic scoring."

How we can help you:

With Litron's MicroFlow kits, you will be using the most validated and peer-reviewed "automated scoring method" available. In fact, it was the MicroFlow method that was used for the comparative studies referred to in the guideline (Reference 1, Reference 2). This method is used by 16 of the top 20 pharma companies in the world, along with top CRO, medical device and chemical companies, among others.

Since 1999, MicroFlow kits have included "appropriate calibration standards" to ensure inter- and intra-laboratory reproducibility and sensitivity.

More flexibility in dosing and sampling times

OECD updated guideline 474 states, "...If three or more daily treatments are used ... peripheral blood should be collected no later than 40 hours after the last treatment" [emphasis added]

"This treatment option accommodates combination of the comet assay ...and integration of the micronucleus test with repeated-dose toxicity studies."

How we can help you:

MicroFlow kits are particularly well suited to take advantage of this flexibility. With only a few drops of blood you can easily integrate micronucleus analyses into your existing studies.