MicroFlow In Vitro 2,000/400 Kit

Size of Kit

2,000 samples (96 well plate format) or 400 samples (standard format - i.e., flasks or plate formats with fewer than 96 wells)

Shipping Conditions of the Kit

• Ambient

Storage Conditions of Kit Components

• Freezer (-20 °C)
• Refrigerator (4 °C)
• Ambient

What's Included in the Kit

• Incomplete Lysis Solution 1
  Lyses cells after staining
• Incomplete Lysis Solution 2
  Lyses cells after staining
• Nucleic Acid Dye A
  Stains the DNA of cells undergoing apoptosis or necrosis
• Nucleic Acid Dye B
  Stains nuclei and micronuclei
• RNase Solution
  Removes RNA so Nucleic Acid Dye B stains only nuclei and micronuclei
• 10x Buffer
  Used in the lysis solutions

Additional Materials Required

• Cell line and cell culture materials
• Freezer (-20 °C) and refrigerator (4 °C)
• Centrifuge and other general lab supplies
• Flow cytometer capable of 488 nm excitation
• Light source (fluorescent preferred)
• If using 96-well plates, an 8-channel bridge and aspirator manifold (available from V&P Scientific)