MultiFlow – p53 Kit

Analyze in vitro samples for p53 DNA damage response pathways using your own flow cytometer

Study an endpoint associated with DNA damage response pathways. A p53 antibody labels nuclei with activated (translocated) p53 protein, an important mediator of genotoxic and other stress responses.

Product ID: MultiFlow (p53)

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Technical Specifications

Size of Kit

384 tests

Shipping Conditions of the Kit

Ambient

Storage Conditions of Kit Components

Refrigerator (4 °C)
Ambient

What's Included in the Kit

Nuclei Release Solution with Counting Beads
Lyses cells and provides absolute bead count
DNA Stain
Labels free nuclei
RNase Solution
Removes RNA
p53 Antibody FITC
Detects nuclear translocation of p53

Additional Materials Required

Human cell line (ideally with functional p53) and cell culture materials
Refrigerator (4 °C)
Centrifuge and other general lab supplies
Accutase^® cell detachment solution if working with attachment cells
Flow cytometer capable of 488 nm and 647 nm excitation, optionally equipped with High Throughput Sampler (HTS) or similar autosampler