MultiFlow DNA Damage Kit – p53, γH2AX, Phospho-Histone H3, Cleaved PARP Kit

Analyze in vitro samples for DNA damage using your own flow cytometer

Study multiple endpoints associated with DNA damage response pathways. These endpoints include p53 as an indicator of nuclear p53, γH2AX as a measure of DNA double strand breaks, phospho-histone H3 to label mitotic cells, and cleaved PARP as an apoptosis marker.

Product ID: MultiFlow (p53, γH2AX, H3, PARP)

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Technical Specifications

Size of Kit

384 tests

Shipping Conditions of the Kit

Ambient

Storage Conditions of Kit Components

Refrigerator (4 °C)
Ambient

What's Included in the Kit

Nuclei Release Solution with Counting Beads
Lyses cells and provides absolute bead count
DNA Stain
Labels free nuclei
RNase Solution
Removes RNA
p53 Antibody FITC
Detects nuclear translocation of p53
γH2AX Antibody Alexa Fluor^® 647
Detects double-strand breaks
Phospho-Histone H3 Antibody PE
Detects mitotic cells
Cleaved PARP Antibody Violet
Detects apoptotic chromatin

Additional Materials Required

Human cell line (ideally with functional p53) and cell culture materials
Refrigerator (4 °C)
Centrifuge and other general lab supplies
Accutase^® cell detachment solution if working with attachment cells
Flow cytometer capable of 405 nm, 488 nm, and 647 nm excitation, optionally equipped with High Throughput Sampler (HTS) or similar autosampler