MultiFlow DNA Damage Kit – p53, γH2AX, Phospho-Histone H3, Cleaved PARP Kit

Analyze in vitro samples for DNA damage using your own flow cytometer

Study multiple endpoints associated with DNA damage response pathways. These endpoints include p53 as an indicator of nuclear p53, γH2AX as a measure of DNA double strand breaks, phospho-histone H3 to label mitotic cells, and cleaved PARP as an apoptosis marker.

Product ID: MultiFlow (p53, γH2AX, H3, PARP)

Would you like to add micronucleus data to your study?

Click here for the MicroFlow In Vitro 2,000/400 kit.

Would you like to measure mode of action in non-human cell lines?

Click here for the MultiFlow (PARP, γH2AX, H3) kit.

Would you like to measure apoptosis?

Click here for the MultiFlow Cleaved-PARP kit.

Technical Specifications

Size of Kit

384 tests

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • Nuclei Release Solution with Counting Beads
  • Lyses cells and provides absolute bead count
  • DNA Stain
    Labels free nuclei
  • RNase Solution
    Removes RNA
  • p53 Antibody FITC
    Detects nuclear translocation of p53
  • γH2AX Antibody Alexa Fluor® 647
    Detects double-strand breaks
  • Phospho-Histone H3 Antibody PE
    Detects mitotic cells
  • Cleaved PARP Antibody Violet
    Detects apoptotic chromatin

Additional Materials Required

  • Human cell line (ideally with functional p53) and cell culture materials
  • Refrigerator (4 °C)
  • Centrifuge and other general lab supplies
  • Accutase® cell detachment solution if working with attachment cells
  • Flow cytometer capable of 405 nm, 488 nm, and 647 nm excitation, optionally equipped with High Throughput Sampler (HTS) or similar autosampler

Resources

Questions & Answers

  • Answers provided by the same scientists who developed the assay. Click here.
  • Or ask your own question about our kits!