MicroFlow PLUS-RBM Kit

Size of Kit

60 rat bone marrow samples

Shipping Conditions of the Kit

• Ambient
• Ice packs (Calibration Standards)

Storage Conditions of Kit Components

• Freezer (-80 °C)
• Freezer (-20 °C)
• Refrigerator (4 °C)
• Ambient

What's Included in the Kit

• Long-Term Storage Solution (LTSS)
  Stabilizes fixed samples
• Buffer Solution
  To wash fixed cells for transfer into LTSS and for staining; used to prepare solutions
• RNase Solution
  Removes RNA
• Anti-Rat CD71-FITC Antibody
  Labels young red blood cells
• Anti-Rodent CD61-PE Antibody
  Labels platelets
• DNA Stain
  Stains DNA (e.g., micronuclei)
• Strainer Cap Tubes
  Prevents flow cytometer clogs
• Calibration Standards
  To calibrate flow cytometer

Additional Materials Required

• Bone marrow collection supplies
• Flow cytometer capable of 488 nm excitation
• Fixative (methanol)
• Freezer (-80 °C), freezer (-20 °C), and refrigerator (4 °C)
• Centrifuge and other general lab supplies
• FBS, cellulose, and columns