MultiFlow – Phospho-Histone H3 Kit

Analyze in vitro samples for cellular proliferation using your own flow cytometer

Study an endpoint associated with cellular proliferation. A phospho-histone H3 antibody specifically labels mitotic cells and measures changes to the mitotic index.

Product ID: MultiFlow (H3)
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Would you like to add micronucleus data to your study?

Click here for the MicroFlow In Vitro 2,000/400 kit.

Would you like to measure mode of action in non-human cell lines?

Click here for the MultiFlow (PARP, γH2AX, H3) kit.

Would you like to measure mode of action in human cell lines?

Click here for the MultiFlow (p53, γH2AX, H3) kit.

Technical Specifications

Size of Kit

384 tests

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • Nuclei Release Solution with Counting Beads
  • Lyses cells and provides absolute bead count
  • DNA Stain
    Labels free nuclei
  • RNase Solution
    Removes RNA
  • Phospho-Histone H3 Antibody PE
    Detects mitotic cells

Additional Materials Required

  • Cell line and cell culture materials
  • Refrigerator (4 °C)
  • Centrifuge and other general lab supplies
  • Accutase® cell detachment solution if working with attachment cells
  • Flow cytometer capable of 488 nm and 647 nm excitation, optionally equipped with High Throughput Sampler (HTS) or similar autosampler

Resources

Publications Q & A Support

Instruction Manual

Instruction Manual - In Vitro MultiFlow - Phospho-Histone H3

On-Demand Videos

LEARN 101 In Vitro MultiFlow

Safety Data Sheet (SDS)

Safety Data Sheets (SDS) - MultiFlow - Phospho-Histone H3

Product Sheets

MultiFlow Product Sheet - Family of Kits MultiFlow Product Sheet - Phospho-Histone H3 Kit About Litron In Vitro MultiFlow