MicroFlow In Vitro-250/50 Kit

Analyze in vitro samples for micronuclei using your own flow cytometer

Each MicroFlow In Vitro-250/50 Kit contains all of the quality-assured supplies & reagents to analyze 250 samples in 96 well plates or 50 samples in larger format plates or flasks. May be used with a variety of attachment and suspension cell lines including L5178Y, CHO-K1, TK6, V79, WIL-2, and HepG2, among others.

Product ID: In Vitro-250/50

Would more informaton about double-strand DNA breaks be helpful?

Click here for the MultiFlow γH2AX kit.

Would you like to measure other DNA damage endpoints?

Click here for other MultiFlow kits.

Would you like to analyze even more samples?

Click here for the In Vitro-2,000/400 kit.

Technical Specifications

Size of Kit

250 samples (96 well plate format) or 50 samples (standard format - i.e., flasks or plate formats with fewer than 96 wells)

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Freezer (-20 °C)
  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • Incomplete Lysis Solution 1
    Lyses cells after staining
  • Incomplete Lysis Solution 2
    Lyses cells after staining
  • Nucleic Acid Dye A
    Stains the DNA of cells undergoing apoptosis or necrosis
  • Nucleic Acid Dye B
    Stains nuclei and micronuclei
  • RNase Solution
    Removes RNA so Nucleic Acid Dye B stains only nuclei and micronuclei
  • 10x Buffer
    Used in the lysis solutions

Additional Materials Required

  • Cell line and cell culture materials
  • Freezer (-20 °C) and refrigerator (4 °C)
  • Centrifuge and other general lab supplies
  • Flow cytometer capable of 488 nm excitation
  • Light source (fluorescent preferred)
  • If using 96-well plates, an 8-channel bridge and aspirator manifold (available from V&P Scientific)


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