In Vitro High Content Assays

(MultiFlow Family of Kits)

Measuring Multiple Endpoints
in One Step

Exposure to a test substance can cause toxicity such as DNA damage or apoptosis. The MultiFlow™ family of kits provides a platform for quickly detecting this information.

These kits were developed from the ground up to be simple and efficient. With this method, you can now liberate nuclei, stain nucleic acids, and label nuclear epitopes all in the same step. After a brief incubation period, samples are ready for flow cytometric analysis.

Article Selected as Editor's Choice

Litron's recent paper in Environmental & Molecular Mutagenesis is available as Open Access. This article describes the interlaboratory validation of our new high information content MultiFlow assay.

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Unlimited Technical Support

Speak with the scientists who designed the method

Unlimited phone and email technical support (send us your plots!)

Training, at your site or ours

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Features

  • One Step, Multiple Endpoints
    Flexible method addresses different pathways and events.
  • Low Compound Requirements
    Perform analyses in 96-well plates.
  • No Washes Required
    Add-and-read format lyses cells and stains nuclei in one step.
  • QC’d Kits, Unlimited Technical Support
    Everything you need to successfully perform the method is included in the box or available for download!

Benefits

  • Easy to Use Multiplexed Assay
    High information content yields Mode of Action data.
  • Place Anywhere In Your Pipeline
    Use as a screening tool or a follow up to a positive result.
  • Fast Results, Reliable Data
    Speed up your work with efficient, rapid processing and analysis.
  • Feel Confident
    Speak with the scientists who developed this method. Send plots, email, or call with questions!

Single Antibody Kits

Each single antibody kit provides
cell density, proliferation and cytotoxicity information.

Additionally, they measure:

Double-stranded DNA breaks using γH2AX

Genotoxic stress using p53

Mitotic cells using phospho-histone H3

Apoptosis using cleaved-PARP

More kits under development

Multiple Antibody Kits

Each multiple antibody kit provides
cell density, proliferation and cytotoxicity information.

Additionally, they provide:

p53, γH2AX, and phospho-histone H3
(for detection of genotoxic stress, double-stranded DNA breaks, and mitotic cells)

Cleaved-PARP, γH2AX and phospho-histone H3
(for detection of apoptosis, double-stranded DNA breaks, and mitotic cells)

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