MicroFlow PLUS-RL Kit

Analyze rat liver samples for micronuclei using your own flow cytometer

Each MicroFlow PLUS-RL kit contains all of the quality-assured supplies & reagents to collect and process 25 rat liver samples. With this MicroFlow PLUS kit, analysis are performed using your own in-house flow cytometer.

Product ID: PLUS-RL

Would you like to add mutation data to your study?

Click here for the MutaFlow PLUS-25R kit.

Would you like to analyze mouse liver instead?

Click here for the PLUS-ML kit.

Would you like to analyze rat blood instead?

Click here for the PLUS-R kit.

Technical Specifications

Size of Kit

25 rat liver samples

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Freezer (-20 °C)
  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • 10X Liver Rinse
    Rinses livers before collagenase treatment
  • EDTA Solution
    Stabilizes livers after removal
  • EGTA Solution
    Removes red blood cells from livers
  • Collagenase Solution
    Digests collagen to break up liver cells
  • Incomplete Liver Lysis Solution 1
    Lyses cells during staining
  • Incomplete Liver Lysis Solution 2
    Lyses cells during staining
  • Ki-67 Antibody eFluor 660
    Labels dividing cells
  • Liver Nucleic Acid Dye
    Stains nuclei and micronuclei
  • RNase Solution
    Removes RNA so Liver Nucleic Acid Dye Stains only nuclei and micronuclei

Additional Materials Required

  • Hanks' Balanced Salt Solution without calcium and magnesium
  • Dimethyl Sulfoxide
  • Freezer (-20 °C) and refrigerator (4 °C)
  • Centrifuge, heat-inactivated fetal bovine serum, and other general lab supplies
  • Shaking incubator
  • Flow cytometer capable of 488 nM and far red excitation (e.g., 633 nM)
  • Vortex mixer (preferably with a vertical six 50 mL tube holder)
  • 5 mL round-bottom tubes with 35 micron cell strainer caps


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