MicroFlow PLUS-ML Kit

Size of Kit

25 mouse liver samples

Shipping Conditions of the Kit

• Ambient

Storage Conditions of Kit Components

• Freezer (-20 °C)
• Refrigerator (4 °C)
• Ambient

What's Included in the Kit

• 10X Liver Rinse
  Rinses livers before collagenase treatment
• EDTA Solution
  Stabilizes livers after removal
• EGTA Solution
  Removes red blood cells from livers
• Collagenase Solution
  Digests collagen to break up liver cells
• Incomplete Liver Lysis Solution 1
  Lyses cells during staining
• Incomplete Liver Lysis Solution 2
  Lyses cells during staining
• Ki-67 Antibody eFluor 660
  Labels dividing cells
• Liver Nucleic Acid Dye
  Stains nuclei and micronuclei
• RNase Solution
  Removes RNA so Liver Nucleic Acid Dye Stains only nuclei and micronuclei

Additional Materials Required

• Hanks' Balanced Salt Solution without calcium and magnesium
• Dimethyl Sulfoxide
• Freezer (-20 °C) and refrigerator (4 °C)
• Centrifuge, heat-inactivated fetal bovine serum, and other general lab supplies
• Shaking incubator
• Flow cytometer capable of 488 nM and far red excitation (e.g., 633 nM)
• Vortex mixer (preferably with a vertical six 50 mL tube holder)
• 5 mL round-bottom tubes with 35 micron cell strainer caps