MutaFlow Mouse Blood Thawing and Analysis Kit

Size of Kit

25 mouse blood samples

Shipping Conditions of the Kit

• Ambient

Storage Conditions of Kit Components

• Freezer (-20 °C)
• Refrigerator (4 °C)
• Ambient

What's Included in the Kit

• Rodent Blood Thawing Solution A
  First solution used to thaw blood samples
• Rodent Blood Thawing Solution B
  Second solution used to thaw blood samples
• Anticoagulant Solution
  Dilutes blood and prevents clotting
• Buffered Salt Solution
  To wash cells and used to prepare solutions
• Anti-Rat CD24-PE Antibody
  Labels wild-type cells
• Anti-CD45-PE Solution
  Labels leukocytes
• Anti-Rodent CD61-PE Antibody
  Labels platelets
• Nucleic Acid Dye Solution
  Stains nuclei and differentiates immature RBCs from mature RBCs from leukocytes

Additional Materials Required

• Previously prepared frozen (-80 °C) mouse blood samples
• Freezer (-20 °C), and refrigerator (4 °C)
• Centrifuge, heat-inactivated fetal bovine serum, and other general lab supplies
• Flow cytometer capable of 488 nm excitation
• Lympholyte-Mammal^® from Cedarlane Laboratories
• LS Columns and either MidiMACS^® or QuadroMACS^® separators from Miltenyi Biotec
• Anti-PE MicroBeads from Miltenyi Biotec
• CountBright™ Absolute Counting Beads from Life Technologies