MutaFlow In Vitro 150 Kit

Measure Pig-a gene mutation in cell lines using your own flow cytometer

Each MutaFlow In Vitro 150 Kit contains all of the quality-assured supplies & reagents to process and analyze 150 samples using your own cell lines and in-house flow cytometer. Litron currently only supports the use of the L5178Y cell line with this kit.

Product ID: MutaFlow In Vitro 150 Kit
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Would you like to add micronucleus data to your study?

Click here for In Vitro MicroFlow kits.

Would you like to measure DNA damage endpoints?

Click here for MultiFlow kits.

Would you like to measure in vivo Pig-a gene mutations?

Click here for In Vivo MutaFlow kits.

Technical Specifications

Size of Kit

150 Samples (96-well plate format)

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Freezer (-20 °C)
  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • 1X Buffer Solution
    Washes cells out of antibody solution
  • Anti-CD45-FITC Antibody
    Labels all cells
  • Anti-CD90.2-PE Antibody
    Labels wild type cells
  • Isotype Control Antibody
    Mimics mutant cells

Additional Materials Required

  • Cell line L5178Y/Tk+/- 3.7.2C and appropriate growth medium
  • CO2-regulated, 37 °C incubator
  • -20 °C Freezer
  • 4 °C Refrigerator
  • Centrifuge and other general lab supplies
  • Vortex Mixer
  • Heat-inactivated fetal bovine serum (FBS)
  • Flow cytometer equipped with 488 and 633 nm excitation sources
  • Optional: DRAQ7 Solution

Resources

Publications Q & A Support

Instruction Manual

Instruction Manual - In Vitro MutaFlow

Product Sheets

About Litron