MutaFlow In Vitro 200 Kit

Measure Pig-a gene mutation in cell lines using your own flow cytometer

Each MutaFlow In Vitro 200 Kit contains all of the quality-assured supplies & reagents to process and analyze 200 samples using your own cell lines and in-house flow cytometer. Litron currently only supports the use of the L5178Y cell line with this kit.

Product ID: MutaFlow In Vitro 200 Kit

Would you like to add micronucleus data to your study?

Click here for in vitro MicroFlow kits.

Would you like to measure other DNA damage endpoints?

Click here for other MultiFlow kits.

Would you like to measure in vivo Pig-a gene mutations?

Click here for in vivo Pig-a gene mutation

Technical Specifications

Size of Kit

200 Samples (96-well plate format)

Shipping Conditions of the Kit

  • Ambient

Storage Conditions of Kit Components

  • Freezer (-20 °C)
  • Refrigerator (4 °C)
  • Ambient

What's Included in the Kit

  • Hank's balanced salt solution (1x)
  • Anti-CD-45-FITC Antibody
  • Anti-CD-90.2-PE Antibody
  • Isotype Control Antibody

Additional Materials Required

  • DRAQ7 Solution, Cell Signaling Technologies Part #7406 recommended
  • Cell line L5178Y/Tk+/- 3.7.2C and appropriate growth medium
  • Sterile fetal bovine serum (FBS)
  • Filter-sterilized deionized water (dH2O)
  • Freezer (approximately –10 °C to –30 °C)
  • Refrigerator (approximately 2 °C to 8 °C)
  • Centrifuge
  • Dimethyl sulfoxide (DMSO)
  • Cell culture supplies – T25 flasks, etc
  • Disposable pipettes sized 5, 10, 25, and 50 mL
  • Vortex Mixer
  • Polypropylene centrifuge tubes (for instance 15 mL) • Micropipettors (20 μL - 1000 μL) and tips
  • CO2-regulated, 37 °C incubator
  • Flow cytometer equipped with 488 and 633 nm excitation sources
  • Flow cytometry tubes

Resources

Questions & Answers

  • Answers provided by the same scientists who developed the assay. Click here.
  • Or ask your own question about our kits!