Litron has a long history of using flow cytometry to automate challenging assays. Today, we've taken another step forward in our continuing effort to provide the genetox community with a flow cytometric liver micronucleus assay.
In addition to detecting changes in hepatocyte proliferation, this assay can detect micronucleus increases in the liver even when they’re not detected in blood. Two new publications describing this process are now available:
- Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation. SL Avlasevich, S Khanal, P Singh, D Torous, J Bemis, S Dertinger (2018) Environmental and Molecular Mutagenesis, Early View.
- Integration of liver and blood micronucleus and Pig-a gene mutation endpoints into rat 28-day repeat-treatment studies: Proof-of-principle with diethylnitrosamine. S Khanal, P Singh, SL Avlasevich, D Torous, J Bemis, S Dertinger (2018) Mutation Research, In Press.
Prototype kits are on their way to beta testers. We're excited to see the results of our hard work in the hands of other scientists!